EliKine Free Thyroxine (fT4) ELISA Kit

SKU: orb3145585

Description

EliKine fT4 ELISA Kit employs a competitive ELISA to quantitate fT4.

Images & Validation

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Tested Applications

ELISA

Application Notes

EliKine fT4 ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT4. A competitive inhibition reaction is launched between fT4 (Standards or samples) and Biotin-conjugated fT4 with the pre-coated antibody specific for fT4. The more amount of fT4 in samples, the less antibody bound by Biotin-conjugated fT4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT4 in the sample. The color development is stopped and the intensity of the color is measured.

Key Properties

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Reactivity	Bovine, Canine, Gallus, Goat, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Sample Types	Serum#Plasma#Other biological fluids
Range	4 pmol/L-64 pmol/L
Sensitivity	4.0 pmol/L

Storage & Handling

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Storage	The unopened kit should be stored at 2 - 8 °C. After opening, please store refer to protocols.
Expiration Date	6 months from date of receipt.
Hazard Information	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Disclaimer	For research use only

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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