ELZ Fushion Kit

The ELZ Kit provides a rapid, high-efficiency directional cloning method based on Gibson Assembly, enabling multiple gene insertions into any vector location without restriction enzyme digestion or multiple cloning sites. Operating the kit is straightforward: mix your linearized vector with PCR fragments bearing 15–40 bp homologous overlaps and incubate at 37°C. The resulting assembly is ready for direct transformation, yielding a positive clone rate of over 95% in just a few hours.