PDI Antibody (RPE)

SKU: orb148149

Description

Mouse monoclonal to EndoPDI (RPE). Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia..

Research Area

Metabolism Research

Images & Validation

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Tested Applications	ELISA, ICC, IF, IHC, IP, WB
Dilution Range	WB (1:200)
Reactivity	Human
Application Notes	1 µg/ml was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	2E7/7
Immunogen	Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
Target	PDI
Molecular Weight	48kDa
Purification	Protein G Purified
Conjugation	RPE

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide isomeras

PDI Antibody (RPE) [orb151399]

Applications:	ICC, IF, IHC, IP, WB
Reactivity:	Bovine, Canine, Frog, Guinea pig, Hamster, Human, Invertebrate, Mouse, Mussel, Porcine, Rat, Sheep
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	RPE

Sizes Available: 100 μl

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