EpCAM Antibody / Extracellular domain

SKU: orb750043

Description

EpCAM (Epithelial Cell Adhesion Molecule), also known as CD326, plays a crucial role in various cellular processes and has been linked to the development and progression of cancer. EpCAM is a transmembrane glycoprotein that is expressed in high levels on the surface of epithelial cells. In cancer cells, the expression of EpCAM is often upregulated, leading to increased cell proliferation, invasion, and metastasis. Studies have shown that high levels of EpCAM are associated with poor prognosis in various types of cancer, including breast, colon, and lung cancer. The overexpression of EpCAM in cancer cells makes it an attractive target for diagnostic and therapeutic purposes. Researchers are exploring the use of EpCAM as a biomarker for early cancer detection through blood tests and imaging techniques. In addition, EpCAM-targeted therapies, such as monoclonal antibodies and immunotherapies, are being developed to specifically target and kill cancer cells that overexpress EpCAM.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, IF, IHC-P, WB
Dilution range	ELISA: order BSA/sodium azide-free format for coating,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Reactivity	Canine, Feline, Guinea pig, Human
Application Notes	Titering of the EpCAM antibody may be required for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG
Clone No.	EGP40/1384
Immunogen	A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the EpCAM antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human ovarian carcinoma and EpCAM antibody (clone EGP40/1384). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

Immunofluorescent staining of human MCF-7 cells with EpCAM antibody (green, clone EGP40/1384) and Reddot nuclear stain (red).

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using EpCAM antibody (clone EGP40/1384). These results demonstrate the foremost specificity of the EGP40/1384 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone EGP40/1384) as confirmation of integrity and purity.

IHC testing of FFPE dog colon and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.

IHC testing of FFPE dog bladder and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.

IHC testing of FFPE cat small intestine and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.

Quick Database Links

UniProt

P16422

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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