Recombinant EpCAM Antibody / Rabbit Monoclonal

SKU: orb750182

Description

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Research Area

Epigenetics

Images & Validation

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Tested Applications	FACS, IF, IHC-P
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
Reactivity	Human
Application Notes	Titering of the recombinant EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	EGP40/1555R
Immunogen	Recombinant full length protein was used as the immunogen for the recombinant EpCAM antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Recombinant EpCAM Antibody / Rabbit Monoclonal

IHC testing of FFPE human prostate carcinoma and recombinant EpCAM antibody (clone EGP40/1555R). HIER: steam sections in pH9 EDTA for 10-20 min.

IHC testing of FFPE human colon carcinoma and recombinant EpCAM antibody (clone EGP40/1555R). HIER: steam sections in pH9 EDTA for 10-20 min.

IHC testing of FFPE human thyroid carcinoma and recombinant EpCAM antibody (clone EGP40/1555R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

Immunofluorescent staining of MeOH fixed human HeLa cells with recombinant EpCAM antibody (clone EGP40/1555R, green) and Reddot nuclear stain (red).

Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/1555R); Red = isotype control, Blue = recombinant EpCAM antibody.

SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/1555R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P16422

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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