EpCAM Rabbit Polyclonal Antibody

SKU: orb1294312

Description

Rabbit polyclonal antibody to EpCAM antibody (intracellular domain)

Research Area

Cancer Biology

Images & Validation

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Item 1 of 7

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:1000–1:2000, Immunofluorescence 1:200–1:500, Immunohistochemistry (Paraffin) 1:200-1:300
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / Intracellular domain corresponding to Human EpCAM
Target	EpCAM / CD326 (Intracellular domain)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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All lanes: Anti-EpCAM antibody - at 1/800 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294312 overnight at 4°C.

Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294312 overnight at 4°C.

Cell: A549, DAPI: 1-300, Phalloidin: 1-300, EpCAM, (orb1294312): 1-200, Anti-rabbit 488: 1-500, NikonEpi-fluorescence (A-ken) 60x.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294312 anti-EpCAM antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294312 at 1/200 RT 1hr.

Quick Database Links

Gene Symbol

EpCAM / CD326 (Intracellular domain)

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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