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EphA1 rabbit pAb
SKU: orb765145
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EPHA1. AA range:541-590 |
| Molecular Weight | 108 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-EPHA1 antibody, anti-EPH antibody, anti-EPHT antibody, anti-EPHT1 antibody, anti-Ephrin type-A receptor 1 antibody, anti-hEpha1 antibody, anti-EPH tyrosine kinase antibody, anti-EPH tyrosine kinase 1 antibody, anti-Erythropoietin-producing hepatoma receptor antibody, anti-Tyrosine-protein kinase receptor EPH antibody
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Western Blot analysis of various cells using EphA1 Polyclonal Antibody.
Immunofluorescence analysis of HeLa cells, using EPHA1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO205 cells, using EPHA1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
EphA1 rabbit pAb (orb765145)
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