EphA2 Antibody (N-term)

SKU: orb1929104

Description

Images & Validation

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Item 1 of 6

Tested Applications	FC, IHC-P, WB
Dilution range	WB - 1:1000, FC - 1:10-50, IHC-P - 1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Monkey

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	108266 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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ECK

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Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates (35 ug/lane). EPHA2 (arrow) was detected using the purified Pab

Western blot analysis of hEPHA2-T45 in mouse NIH-3T3 cell line lysates (35 ug/lane). EPHA2 (arrow) was detected using the purified Pab.

Western blot analysis of EPHA2 (arrow) using rabbit polyclonal hEPHA2-T45. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)

Flow cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-EPHA2 Antibody (T45) at 1:1000 dilution + MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 108 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

UniProt

P29317

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Flow Cytometry

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