EphA4 Antibody (N-term)

SKU: orb1929100

Description

Images & Validation

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Item 1 of 5

Tested Applications	FC, IF, IHC-P, WB
Dilution range	WB - 1:1000, IF - 1:100, FC - 1:10-50, IHC-P - 1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Gallus, Xenopus

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	109860 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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HEK8, SEK, TYRO1

Quality Guarantee

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Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endothelial Lectin (red).

Western blot analysis of EphA4 (arrow) using EphA4 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA4 gene (Lane 2).

Flow cytometric analysis of CEM cells using EphA4 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-EPHA4 Antibody (E55) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: NCI-H460 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

UniProt

P54764

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence