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ERK1/2 Antibody
SKU: orb626803
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | WB:1:500-1:5000; IHC: 1:100-1:400; IF: 1:20-1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | mitogen-activated protein kinase 3 |
| Target | ERK1/2 |
| Molecular Weight | 38-44 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
Alternative Names
−ERK, ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3
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Immunofluorescent analysis of HepG2 cells using orb626803 (ERK1/2 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
HeLa cells were subjected to SDS PAGE followed by western blot with orb626803 (ERK1/2 Antibody) at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using orb626803 (ERK1/2 Antibody) at dilution of 1:200. Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.
Quick Database Links
Gene Symbol
ERK1/2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ERK1/2 Antibody (orb626803)
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