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KO/KD
Validated
Validated
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | KO/KD Validated, WB |
|---|---|
| Dilution Range | WB - 1:1000 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Other, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone No. | B036EV7X6X4 |
| Immunogen | This Erk2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from human Erk2. Antigen Region: 154-183 aa. |
| Target | MAPK1 (HGNC:6871) |
| Molecular Weight | 41390 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2
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MAPK1 Antibody (Center) western blot analysis in MCF-7, HepG2, NCI-H292, Hela, mouse NIH/3T3 and Neuro-2a, rat C6 cell line lysates (35μg/lane). This demonstrates the MAPK1 antibody detected the MAPK1 protein (arrow).
All lanes: Anti-MAPK1 Antibody at 1:1000 dilution (upper). Lane 1: Hela. Lane 2: Hela-MAPK1-Knock out. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-MAPK1 Antibody (Center) at 1:1000 dilution. Lane 1: NCI-H292 whole cell lysate. Lane 2: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 41KDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_620407.1, NP_002736.3 |
|---|
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Erk2 Antibody (orb1925218)
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