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Recombinant Estrogen Receptor Antibody (alpha)
SKU: orb2640594
Description
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P |
|---|---|
| Dilution Range | The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | Rabbit IgG, kappa |
| Clone No. | ESR1/2299R |
| Immunogen | Human Estrogen Receptor alpha protein was used as the immunogen for this recombinant Estrogen Receptor antibody. |
| Purification | Protein A affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC testing of FFPE human breast cancer with Estrogen Receptor antibody (clone ESR1/2299R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Estrogen Receptor antibody (clone ESR1/2299R) as confirmation of integrity and purity.
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Recombinant Estrogen Receptor Antibody (alpha) (orb2640594)
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