Fab Mouse IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb348472

Description

Fab Mouse IgG (H&L) antibody (FITC)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, IF, WB
Dilution range	IF: 1:500 - 1:2,500
Reactivity	Mouse
Application Notes	Fab Anti-Mouse IgG (H&L) Fluorescein Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG Fab
Immunogen	Mouse IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat Fab Anti-Mouse IgG Antibody Fluorescein Conjugation, Goat Fab Anti-Mouse IgG FITC Conjugated Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Fab Mouse IgG (H&L) Antibody Fluorescein Conjugated

Dot Blot results of Goat Fab Anti-Mouse IgG Antibody Fluorescein Conjugated. Dots are Mouse IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Goat Anti-Mouse IgG FITC at 1 µg/ml for 1hr at RT. Secondary Antibody: none.

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Western Blot (IB, immunoblot)

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Immunofluorescence

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DOT

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