F(ab')2 Bovine IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb348075

Description

F(ab')2 Bovine IgG (H&L) antibody (FITC)

Images & Validation

−

Item 1 of 1

Tested Applications	DOT, FC, FLISA, IF
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Bovine
Application Notes	F(ab')2 Anti-Bovine IgG Fluorescein conjugated antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

−

Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Bovine IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.
Conjugation	FITC

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

−

Rabbit F(ab')2 Anti-Bovine fluorescein Conjugated Antibody, Rabbit Fab2 Anti-Bovine fluorescein Conjugated Antibody, Rabbit Fab'2 Anti-Bovine Antibody FITC Conjugation

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

F(ab')2 Bovine IgG (H&L) Antibody Fluorescein Conjugated

Dot Blot results of Rabbit F(ab')2 Anti-Bovine IgG Antibody Fluorescein Conjugated. Dots are Bovine IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Rabbit F(ab')2 Anti-Bovine IgG Antibody FITC at 1 µg/ml for 1hr at RT. Secondary Antibody: none.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Flow Cytometry

View Protocol

Immunofluorescence

View Protocol

DOT

Dot Blot

View Protocol

Find More Protocols