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F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed
SKU: orb348407
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:5000 - 1:25,000, IHC: 1:300-1:2000, WB: 1:2,000-1:10,000 |
| Reactivity | Golden Hamster, Hamster |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | Golden Syrian and Armenian Hamster IgG whole molecules |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian and Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Golden Syrian and Armenian Hamster IgG and Golden Syrian and Armenian Hamster Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) and Mouse or Rat Serum Proteins. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Goat F(ab')2 Anti-Hamster IgG Antibody Biotin Conjugation, Goat Fab2 Anti-Hamster IgG biotin Conjugated Antibody, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Biotin Conjugation, Goat Fab2 Anti-Golden Syrian & Armenian Hamster IgG biotin Conjugated Antibody
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ELISA results of purified F(ab')2 Fragment of GOAT Anti-GOLDEN SYRIAN & ARMENIAN HAMSTER IgG Biotin Conjugated Antibody tested against purified GOLDEN SYRIAN & ARMENIAN HAMSTER IgG. Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin HRP conjugate 1:10000.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed (orb348407)
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