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F(ab')2 Human IgG Fc Pre-adsorbed Phycoerythrin Conjugated Antibody
SKU: orb348205
Description
Images & Validation
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| Tested Applications | DOT, FC, IF, WB |
|---|---|
| Dilution Range | FC: 1:100 - 1:250, IF: 1:100 - 1:250 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | Anti-Human IgG was produced by repeated immunization with Human IgG F(c) fragment in goat. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab) or Bovine, Horse, Mouse and Rat Serum Proteins. |
| Conjugation | RPE |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Goat F(ab')2 Anti-Human IgG F(c) Phycoerythrin Conjugated Pre-Adsorbed Antibody, Goat Fab2 Anti-Human IgG Fc Fragment Phycoerythrin Conjugated Antibody, Goat F(ab')2 Anti-Human IgG F(c) Fragment Antibody PE Conjugated
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
DOT
Dot Blot
Available Sizes
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Usually dispatched within 5-10 working days