F(ab')2 Human IgG (H&L) Antibody Texas Red Conjugated Pre-Adsorbed

SKU: orb348209

Description

F(ab')2 Human IgG (H&L) antibody (Texas Red)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, FC, FLISA, IF
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Human
Application Notes	F(ab')2 Anti-Human IgG (H&L) Texas Red Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Human IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins.
Conjugation	Texas Red

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Human IgG Texas Red Conjugated Antibody Pre-Adsorbed, Goat F(ab')2 Anti-Human IgG Antibody Texas Red Conjugation

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

F(ab')2 Human IgG (H&L) Antibody Texas Red Conjugated Pre-Adsorbed

Dot Blot results of Goat F(ab')2 Anti-Human IgG Antibody Texas Red™ Conjugated. Dots are Human IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: Goat F(ab')2 Anti-Human IgG Antibody Texas Red™ at 1 µg/ml for 1hr at RT. Secondary Antibody: none.

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