F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

SKU: orb348280

Description

F(ab')2 MOUSE IgG (H&L) antibody (RPE)

Images & Validation

−

Item 1 of 1

Tested Applications	DOT, FC, IF
Dilution range	FC: 1:100 - 1:250, IF: 1:100 - 1:250
Reactivity	Mouse
Application Notes	F(ab')2 Mouse IgG Antibody Phycoerythrin Conjugated Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Read more...

Key Properties

−

Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in rabbit.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Conjugation	RPE

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

−

Rabbit F(ab')2 Anti-Mouse IgG Antibody phycoerythrin Conjugation, Rabbit Fab2 Anti-Mouse IgG PE Conjugated Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Dot Blot results of Rabbit F(ab')2 Anti-Mouse IgG Antibody Phycoerythrin Conjugated. Dots are Mouse IgG at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Rabbit F(ab')2 Anti-Mouse IgG Antibody RPE at 1 µg/ml for 1hr at RT.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Flow Cytometry

View Protocol

Immunofluorescence

View Protocol

DOT

Dot Blot

View Protocol

Find More Protocols