F(ab')2 Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb348297

Description

F(ab')2 Rabbit IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:2,000 - 1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
Reactivity	Rabbit
Application Notes	F(ab')2 Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Rabbit IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody alk phos Conjugation

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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