F(ab')2 Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

SKU: orb348305

Description

F(ab')2 Rabbit IgG (H&L) antibody (FITC)

Images & Validation

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Item 1 of 2

Tested Applications	DOT, FC, FLISA, IF, WB
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Rabbit
Application Notes	F(ab')2 Anti-Rabbit IgG Fluorescein Conjugated Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Rabbit IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Goat F(ab')2 Anti-Rabbit IgG Fluorescein Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody FITC Conjugation

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

F(ab')2 Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Dot Blot of Goat Fab2 anti-Rabbit IgG Antibody Fluorescein Conjugated Pre-Absorbed. Antigen: Rabbit IgG. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat Fab2 anti-Rabbit IgG Antibody Fluorescein Conjugated Pre-Absorbed at 1:1000 for 1 HR at RT. Block: orb348637 for 1 HR at RT.

Western blot of Fluorescein conjugated Goat F(ab')2 Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

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Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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DOT

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