F(ab')2 Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

SKU: orb348323

Description

F(ab')2 Rabbit IgG (H&L) antibody (Peroxidase)

Images & Validation

−

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:8,000 - 1:40,000, IHC: 1:500 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Rabbit
Application Notes	F(ab')2 Anti-Rabbit IgG Peroxidase Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Key Properties

−

Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Rabbit IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Conjugation	HRP

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

−

Donkey F(ab')2 Anti-Rabbit IgG Peroxidase Conjugated Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody HRP Conjugation

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols