F(ab')2 Rat IgG F(ab')2 Antibody Fluorescein Conjugated

SKU: orb346797

Description

F(ab')2 Rat IgG F(ab')2 antibody (FITC)

Images & Validation

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Tested Applications	FC, FLISA, IF
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Rat
Application Notes	F(ab')2 Anti-Rat IgG F(ab')2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Rat IgG F(ab')2 fragment
Purity	This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Thimerosal. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	10.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit F(ab')2 anti-Rat IgG F(ab')2 Fluorescein Conjugated Antibody, rabbit F(ab')2 anti-Rat IgG F(ab')2 FITC Conjugated Antibody

Rat IgG F(ab')2 Fluorescein Antibody [orb346344]

Conjugation:	FITC
Purity:	This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Pepsin.
Source:	Rat

Sizes Available: 1 mg

Item 1 of 1
Rat IgG F(ab')2 Antibody Fluorescein Conjugated [orb347855]
Applications:
FC, FLISA, IF
Reactivity:
Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
FITC
Sizes Available: 2 mg
Item 1 of 1
F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed [orb348342]
Applications:
FC, FLISA, IF, WB
Reactivity:
Rat
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
FITC
Sizes Available: 0.5 mg

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