F(ab')2 Swine IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb346807

Description

F(ab')2 Swine IgG (H&L) antibody (FITC)

Images & Validation

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Item 1 of 1

Tested Applications	FC, FLISA, IF, WB
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Porcine
Application Notes	F(ab’)2 Anti-Swine IgG Antibody Fluorescein Conjugate has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Swine IgG whole molecule
Purity	This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Swine IgG and Swine Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Thimerosal
Concentration	10.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit F(ab')2 anti-swine IgG Fluorescein Conjugated Antibody, rabbit F(ab')2 anti-swine IgG FITC Conjugated Antibody

Quality Guarantee

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F(ab')2 Swine IgG (H&L) Antibody Fluorescein Conjugated

Western Blot of Fluorescein conjugated Rabbit anti-Swine IgG antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein swine secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence