F(ab')2 Swine IgG (H&L) Antibody Alkaline Phosphatase Conjugated

SKU: orb348398

Description

F(ab')2 Swine IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:8,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Porcine
Application Notes	This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG F(ab')2
Immunogen	Swine IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Alkaline Phosphatase, Swine IgG and Swine Serum. No reaction was observed against anti-Pepsin and anti-Rabbit IgG F(c).
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	0.5 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Rabbit F(ab')2 Anti-Swine IgG Antibody Alkaline Phosphatase Conjugation, Rabbit F(ab')2 Anti-Swine IgG alk phos Conjugated Antibody

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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