FABP4 Recombinant Rabbit Monoclonal Antibody

SKU: orb1152150

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Description

Research Area

Adipogenesis, Binding Proteins, Cancer Research, Cell Biology, Fatty Acid Oxidation, Fatty Acids, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism, cell Biology

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	F12H4
Immunogen	KLH conjugated synthetic peptide derived from human FABP4
Target	FABP4
Molecular Weight	14 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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adipocyte; 3T3-L1 lipid-binding protein; 422/aP2; A FABP; A-FABP; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; Adipocyte protein AP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBPv ALBP/Ap2; aP2; FABP; FABP4_HUMAN; Fatty acid binding protein 4 adipocyte; Fatty acid binding protein 4; Fatty acid binding protein adipocyte; Fatty acid-binding protein 4; Fatty acid-binding protein; Lbpl; Myelin P2 protein homolog; P15v P2 adipocyte protein; Protein 422.

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FABP4 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-FABP4 antibody (orb1152150) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1152150) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Sample: Lane 1: Mouse Adipose tissue lysates, Lane 2: Mouse Breast tissue lysates, Lane 3: Mouse Heart tissue lysates, Lane 4: Mouse Thymus tissue lysates, Primary: Anti-FABP4 (orb1152150) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kDa, Observed band size: 14 kDa.