Factor VIIIa light chain Rabbit Polyclonal Antibody

SKU: orb156822

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Description

Factor VIIIa light chain Rabbit Polyclonal Antibody. FVIII is a large molecule glycoprotein composed of 2,332 amino acids and is divided into multiple functional domains (arranged in the order of A1-A2-B-A3-C1-C2).

Images & Validation

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Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Human

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Factor VIIIa light chain (1751-1850/2351aa)
Target	F8
Molecular Weight	75 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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coagulation factor VIII; Ahf; Antihemophilic factor; Coagulation factor VIII; Coagulation factor VIII associated protein b; Coagulation factor VIII isoform b; Coagulation factor VIII procoagulent component; Coagulation factor VIIIc; Dna segment on chromosome x unique 1253 expressed sequence; Dxs1253e; F8; F8 protein; F8b; F8c; FactorVIII; FVIII; Hema; Hema coagulation factor VIIIc procoagulent component; Hemophilia a; Hemophilia classic; OTTHUMP00000061446; Procoagulant component; AHF; DXS1253E; F8B; F8C; FVIII; HEMA; FA8_HUMAN.

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Factor VIIIa light chain Rabbit Polyclonal Antibody

Sample: Lymph node (Mouse) Lysate at 40 ug, Testis (Mouse) Lysate at 40 ug, Primary: Anti-Factor VIIIa light chain (orb156822) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 75 kD, Observed band size: 75 kD.

Sample: Raji (human) cell Lysate at 30 ug, Primary: Anti-Factor VIIIa light chain (orb156822) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 75kD, Observed band size: 85kD.

Quick Database Links

Gene Symbol

UniProt

P00451

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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