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Fc gamma receptor III Antibody [3G8], Rabbit IgG
SKU: orb1671874
Description
Research Area
Cancer Biology, Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | Blocking, Depletion, FC, IHC, IP |
|---|---|
| Reactivity | Human, Marmoset, Monkey, Primate |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | IgG kappa |
| Clone No. | 3G8 |
| Immunogen | 3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD16, low affinity IgG receptor III, FcγRIII, low affinity FcγRIII
Similar Products
−- Item 1 of 2
Anti-Fc gamma receptor III [3G8] [orb613919]
Blocking, FC, IHC, IP
Human, Marmoset, Primate
Mouse
Monoclonal
Unconjugated
0.2 mg
![Anti-Fc gamma receptor III [3G8]](/images/pub/media/catalog/product/NewWebsite/35/orb613919_1.png)
![Anti-Fc gamma receptor III [3G8]](/images/pub/media/catalog/product/NewWebsite/35/orb613919_2.png)
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![Fc gamma receptor III Antibody [3G8], Rabbit IgG](/images/pub/media/catalog/product/NewWebsite/15/orb1671874_1.jpg)
Flow-cytometry using the anti-Fc gamma receptor III antibody 3G8. Human peripheral blood leukocytes were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control - black line) or the r-chimeric version of 3G8 (orb1671874 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
![Fc gamma receptor III Antibody [3G8], Rabbit IgG](/images/pub/media/catalog/product/NewWebsite/15/orb1671874_2.jpg)
Immunofluorescence staining of fixed U937 cells with anti-Fc gamma receptor III antibody 3G8. Immunofluorescence analysis of paraformaldehyde fixed U937 cells permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of 3G8 (orb1671874) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671874- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor® 488 secondary antibody.
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Fc gamma receptor III Antibody [3G8], Rabbit IgG (orb1671874)
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