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Description
Research Area
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 5 ng/ml, corresponding to a specific activity of ≥ 2 x 105 units/mg. |
| Target | FGF8 |
| Purity | Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR |
Storage & Handling
−| Storage | The lyophilized FGF-8 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted FGF-8 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing FGF-8 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human FGF8 Protein, N-His [orb2968325]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
26.5 kDa
100 μg, 1 mg, 50 μgHuman FGF8b(23-215) Protein, hFc Tag [orb2668288]
Unconjugated
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 48.5 kDa after removal of the signal peptide. The apparent molecular mass of FGF8b(23-215)-hFc is approximately 55-70 kDa due to glycosylation.
50 μg, 10 μg, 100 μgHuman FGF8 protein [orb754771]
ELISA, WB
Greater than 95% as determined by SDS-PAGE
21.1 kDa
E.Coli
50 μg, 1 mg, 100 μg, 200 μg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001193318.1 |
|---|
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Protocol Information
FGF-8 Recombinant Protein (orb1230920)
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