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Fibrillarin Recombinant Rabbit Monoclonal Antibody
SKU: orb1172950
Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG/Kappa |
| Clone No. | H4C3 |
| Target | FBL |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; FBRL_HUMAN; FIB; FIB1; FLRN; Nop1p; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2''-O-methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin.
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:3000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HeLa, HepG2, 293T, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 37 kDa, Observed MW: 37 kDa.
Cell line: U-2 OS, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172950.
Quick Database Links
Gene Symbol
FBL
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
Fibrillarin Recombinant Rabbit Monoclonal Antibody (orb1172950)
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