Fibroblasts, Mouse, mAb ER-TR7 Antibody

SKU: orb1109119

Description

Mouse monoclonal antibody to Fibroblasts

Images & Validation

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Tested Applications	FC, IF, IHC-Fr, PS
Dilution Range	For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:20.
Reactivity	Rat
Application Notes	F: Sections were stained using an indirect immunoperoxidase method (Ref.1). FC: Splenocytes were incubated with ER-TR7 for 30'(Ref.4). IF: Acetone or PFA fixed cells were quenched with 50mM NH4Cl for 30', blocked and permeabilized with 1.5% goat serum/0.1% saponin in PBS for 45' at RT. Incubation of ER-TR-7 in block and perm solution for 45' at RT. Specific staining was detected with a fluorescent conjugated oat anti-rat IgG (Ref.5). P: Formalin fixed paraffin sections were deparafined, hydrated in ethanol and stained with ER-TR7 for 3';at RT (Ref.3). Read more...

Key Properties

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Clonality	Monoclonal
Isotype	Rat IgG2a
Immunogen	Mouse thymic stromal cells
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Protocol Information

IHC-Fr

Immunohistochemistry Frozen

Flow Cytometry

Immunofluorescence