FLNA Antibody [Knockout Validated]

SKU: orb1787815

This product can be custom made

KO/KD
Validated

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Signal Transduction

Images & Validation

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Item 1 of 3

Tested Applications	IF, KO/KD Validated, WB
Dilution Range	IF: 1:25, WB: 1:4000, WB: 1:500-1:2000
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Immunogen	Purified His-tagged FLNA protein was used to produced this monoclonal antibody. Antigen Region: 1-360 aa.
Target	FLNA
Molecular Weight	280739 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Filamin-A, FLN-A, Actin-binding protein 280, ABP-280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin, FLNA, FLN, FLN1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

All lanes: Anti-FLNA Antibody at 1:4000 dilution. Lane 1: Hela whole cell lysate. Lane 2: C6 whole cell lysate. Lane 3: PC-3 whole cell lysate. Lane 4: COS-7 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 280 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling FLNA at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

A single >250 kDa band for the Hela wild type lysate was observed (3 ug/ml anti- FLNA) vs the predicted size of 280.7 kDa. The molecular weight discrepancy could be due a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. A weaker band of similar size was observed in the knock out lysate in the 20, 10, and 5 ug lanes, suggesting incomplete knockout of the target gene.