Recombinant FOXA1 Antibody

SKU: orb606445

Description

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate. [UniProt]

Research Area

Developmental Biology, Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	FACS, IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells
Reactivity	Human, Rat
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant FOXA1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rFOXA1/1515
Immunogen	A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this recombinant FOXA1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC testing of FFPE human prostate carcinoma with recombinant FOXA1 antibody (clone rFOXA1/1515). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant FOXA1 antibody (clone rFOXA1/1515) as confirmation of integrity and purity.

FACS testing of PFA-fixed human MCF-7 cells with recombinant FOXA1 antibody (clone rFOXA1/1515); Red = isotype control, Blue = FOXA1 antibody.

Quick Database Links

UniProt

P55317

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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