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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This FOXC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the Central region of human FOXC2. Antigen Region: 159-186 aa. |
| Target | FOXC2 |
| Molecular Weight | 53719 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, MFH-1 protein, Transcription factor FKH-14, FOXC2, FKHL14, MFH1
Quality Guarantee
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FOXC2 Antibody (Center S174) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
FOXC2 Antibody (Center S174) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FOXC2 Antibody (Center S174) for immunohistochemistry. Clinical relevance has not been evaluated.
FOXC2 Antibody (Center S174) western blot analysis in A2058 cell line lysates (15 ug/lane). This demonstrates the FOXC2 antibody detected the FOXC2 protein (arrow).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_005242.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
FOXC2 Antibody (Center S174) (orb1930374)
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