Gab 1 (phospho Tyr659) rabbit pAb

SKU: orb768297

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Description

Rabbit polyclonal antibody to Gab 1 (phospho-Tyr659)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human GAB1 around the phosphorylation site of Tyr659. AA range:644-674
Molecular Weight	76
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-GAB1 antibody, anti-GRB2-associated-binding protein 1 antibody, anti-GRB2-associated binder 1 antibody, anti-Growth factor receptor bound protein 2-associated protein 1 antibody

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Western Blot analysis of 3T3 cells using Phospho-Gab 1 (Y659) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GAB1 (Phospho-Tyr659) Antibody.

Immunofluorescence analysis of HepG2 cell, using GAB1 (Phospho-Tyr659) Antibody. The lane on the right is blocked with the GAB1 (Phospho-Tyr659) peptide.

Immunohistochemistry analysis of paraffin-embedded human breast cancer, using GAB1 (Phospho-Tyr659) Antibody. The picture on the right is blocked with the GAB1 (Phospho-Tyr659) peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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