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GAPDH Mouse Monoclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, FC, IF, IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200 |
| Reactivity | Human, Mouse, Rat, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | glyceraldehyde-3-phosphate dehydrogenase |
| Target | GAPDH |
| Molecular Weight | 36 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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IP Result of anti-GAPDH (IP: orb627236, 4 ug; Detection: orb627236 1:3000) with Hela cells lysate 3000 ug.

HeLa cells were subjected to SDS PAGE followed by western blot with orb627236 (GAPDH antibody) at dilution of 1:10000.

Immunohistochemistry of paraffin-embedded human kidney tissue slide using orb627236 (GAPDH Antibody) at dilution of 1:100.
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Protocol Information
GAPDH Mouse Monoclonal Antibody (orb627236)
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