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GC376 sodium

SKU: orb1302013

Description

GC376 is a potent small molecule inhibitor of 3C-like proteases (3CLpro), exhibiting inhibitory activity in the low micromolar range (IC50 0.49-4.35 µM). It is widely used in biochemical and cellular assays to study coronavirus replication and has demonstrated antiviral efficacy in feline infectious peritonitis models.

Research Area

Infectious Disease & Virology

Images & Validation

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GC376 sodium

Key Properties

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CAS Number1416992-39-6
MW507.53
Purity98.00%
FormulaC21H30N3NaO8S
SMILES[Na+].[H][C@@](CC1CCNC1=O)(NC(=O)[C@H](CC(C)C)NC(=O)OCc1ccccc1)C(O)S([O-])(=O)=O
TargetVirus Protease,SARS-CoV
SolubilityDMSO:45 mg/mL (88.66 mM);5% DMSO+95% Saline:2.25 mg/mL (4.43 mM)

Bioactivity

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Target IC50
3CLpro:0.49~4.35 μM.
In Vivo
GC376 was administered subcutaneously every 12 h at a dose of 15 mg/kg. Results Nineteen of 20 cats treated with GC376 regained outward health within 2 weeks of initial treatment. However, disease signs recurred 1-7 weeks after primary treatment and relapses and new cases were ultimately treated for a minimum of 12 weeks. Relapses no longer responsive to treatment occurred in 13 of these 19 cats within 1-7 weeks of initial or repeat treatment(s) .
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In Vitro
GC376 was significantly effective against caliciviruses (NV and MNV-1), coronaviruses (TGEV, FIPV, MHV, 229E, and BCV), and picornaviruses (HRVs 18, 51, and 68, EV71, and PTV), with nanomolar or low micromolar IC50s, except for FCV and HAV. Interestingly, FCV was less sensitive to GC376, with IC50s of 35 μM, respectively .
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Cell Research
The toxic dose for 50% cell death (TD50) for each compound was determined for the various cells used in this study. Confluent cells grown in 96-well plates were treated with various concentrations (1 to 500 μM) of each compound for 72 h. Cell cytotoxicity was measured by a CytoTox 96 nonradioactive cytotoxicity assay kit and crystal violet staining. The in vitro therapeutic index was calculated by dividing the TD50 by the IC50 .
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Animal Research
GC376 was synthesized in a highly pure form and formulated at a concentration of 53 mg/ml in 10% ethanol and 90% polyethylene glycol 400, as described previously. GC376 was administered subcutaneously (SC) at a dosage of 15 mg/kg q12h SC, unless stated otherwise. The effective dosage for cats with experimentally induced FIP was 10 mg/kg/ q12h SC, but the dosage was raised to 15 mg/kg after the first cat (CT01) failed to respond to a lower dose of 10 mg/kg suggested by earlier pharmacokinetic studies. This was a clinical decision based on this one cat's response to treatment .
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Storage & Handling

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Storagestore under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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3CLpro, GC376 sodium, GC-376 sodium, SARSCoV

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  • GC376 sodium

    GC376 sodium [orb1221168]

    >98% (HPLC)

    1416992-39-6

    507.53

    C21H30N3NaO8S

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Compound Identifiers

PubChem CID
71481119 ↗

Key Properties

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No computed properties available.

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GC376 sodium (orb1302013)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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10 mg
$ 70.00
1 ml x 10 mM (in DMSO)
$ 80.00
25 mg
$ 80.00
50 mg
$ 110.00
100 mg
$ 170.00
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