GCLM Rabbit Polyclonal Antibody

SKU: orb500046

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Description

Research Area

Amino Acid Metabolism, Cancer Research, Cell Biology, Drug Metabolism, Energy Metabolism, Glutamate, Metabolism, Mitochondrial, Mitochondrial Markers, Neuroscience, Oxidative Stress

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human GCLM (31-120/274aa)
Target	GCLM
Molecular Weight	31 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Glutamate-Cysteine Ligase Modifier Subunit; gamma-Glutamylcysteine Synthetase Regulatory Subunit; Glutamate--Cysteine Ligase Modifier Subunit; gamma-ECS Regulatory Subunit; GCS Light Chain; GLCLR; Glutamate-Cysteine Ligase(gamma-Glutamylcysteine Synthetase), Regulatory(30.8kD); Glutamate-Cysteine Ligase Modifier Subunit delta2 Alternative Splicing; Glutamate--Cysteine Ligase Regulatory Subunit; Glutamate-Cysteine Ligase Regulatory Protein; Glutamate-Cysteine Ligase, Modifier Subunit; gamma-Glutamylcysteine Synthetase; GSC Light Chain; GSH0_HUMAN;

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Sample: Hela (Human) Cell Lysate at 30 ug, A673 (Human) Cell Lysate at 30 ug, Primary: Anti-GCLM (orb500046) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-GCLM (orb500046) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 31 kD.

Quick Database Links

Gene Symbol

GCLM

UniProt

P48507

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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