General 5-Hydroxytryptophan (5-HTP) ELISA Kit

SKU: orb781960

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptophan(5-HTP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptophan(5-HTP).

Research Area

Protein Biochemistry

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 400 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HTP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HTP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	All
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	6.25-400 ng/mL
Sensitivity	1.74 ng/mL
Concentration	400 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Oxitriptan ELISA Kit, Cincofarm ELISA Kit, Levothym ELISA Kit, Levotonine ELISA Kit, Oxyfan ELISA Kit, Telesol ELISA Kit, Tript-OH ELISA Kit, Triptum ELISA Kit

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General 5-Hydroxytryptophan (5-HTP) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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