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Description
Research Area
Images & Validation
−| Tested Applications | ELISA |
|---|---|
| Application Notes |
Key Properties
−| Reactivity | All |
|---|---|
| Sample Types | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Type | Competitive |
| Assay Time | 2h |
| Range | 15.63-1000 ng/mL |
| Sensitivity | 5.18 ng/mL |
| Concentration | 1000 ng/mL |
Procedure & Performance
−| Assay Principle | |
|---|---|
| Kit Components | |
| Reagent Preparation | |
| Assay Procedure | |
| Materials Required | |
| Precision | |
| Calculation of Results | |
| Curve Fitting Softwares |
Storage & Handling
−| Storage | Refer to the Storage Guidelines in the Manual |
|---|---|
| Expiration Date | Please enquire. |
| Disclaimer | For research use only |
Alternative Names
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Standard curve for orb781738 ELISA kit.
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General Adenosine Triphosphate (ATP) ELISA Kit (orb781738)
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I recently used Biorbyt’s Universal ATP ELISA Kit in our laboratory and was very satisfied with the overall experience. I highly recommend it to researchers who need quantitative ATP detection. Advantages: Simple and convenient operation: The kit uses a competitive inhibition enzyme immunoassay method with a clear and straightforward workflow. The assay can be completed in approximately 2 hours, making it suitable for routine experiments. The pre-coated ATP plate eliminates many preparation steps. Suitable sensitivity and detection range: The detection range is 15.63–1000 ng/mL, with a sensitivity of approximately 5.18 ng/mL. It effectively covers the ATP concentration requirements of most biological samples, including serum, plasma, cell lysates, and cell culture supernatants. Good reproducibility: The standard curve showed good linearity (the attached figure demonstrates a smooth standard curve), and the CV values between replicate wells were low, indicating reliable and consistent results. Broad applicability: As a universal kit suitable for samples from multiple species, it is highly practical for research areas such as pharmacology, drug discovery, and cellular metabolism. Good cost-effectiveness: Compared with similar products, Biorbyt’s kit is competitively priced and is available in both 48T and 96T formats, making it suitable for experiments of different scales. User experience: In experiments related to cellular energy metabolism, this kit showed stable performance, low background, clear color development, and consistent absorbance readings at 450 nm. The instruction manual was detailed, and technical support was responsive (although I did not encounter any issues during this experiment). Our laboratory is planning to continue purchasing this product. If you are conducting ATP quantification studies, this kit is a reliable choice.
I recently used Biorbyt’s Universal ATP ELISA Kit in our laboratory and was very satisfied with the overall performance. I highly recommend it to researchers who need quantitative ATP detection. Advantages: Simple and convenient operation: The kit uses a competitive inhibition enzyme immunoassay method with a clear and straightforward workflow. The assay can be completed in approximately 2 hours, making it suitable for routine experiments. The ATP-coated plate eliminates the need for extensive preparation steps. Suitable sensitivity and detection range: The detection range is 15.63–1000 ng/mL, with a sensitivity of approximately 5.18 ng/mL, covering the ATP concentration requirements of most biological samples, including serum, plasma, cell lysates, and cell culture supernatants. Good reproducibility: The standard curve showed good linearity (the attached figure demonstrates a smooth standard curve), and the CV values between replicate wells were low, indicating reliable results. Broad applicability: As a universal kit suitable for samples from multiple species, it is highly useful for research areas such as pharmacology, drug discovery, and cellular metabolism. Good cost performance: Compared with similar products, Biorbyt’s kit is competitively priced and is available in both 48T and 96T formats, making it suitable for experiments of different scales. User experience: In cellular energy metabolism studies, this kit showed stable performance, low background, clear color development, and consistent absorbance readings at 450 nm. The instruction manual was detailed, and technical support was responsive (although I did not encounter any issues during this experiment). Our laboratory is planning to continue purchasing this product. For researchers conducting ATP quantification studies, this kit is a reliable choice.
