General Kynurenic Acid (KYNA) ELISA Kit

SKU: orb1946415

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Description

General Kynurenic Acid (KYNA) ELISA Kit

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Application Notes

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with KYNA. During the reaction, KYNA in the sample or standard competes with a fixed amount of KYNA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to KYNA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of KYNA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	All
Sample Types	Serum, Urine, Tissue homogenates, Sample volume: 50μL
Assay Type	Competitive
Assay Time	1.5H
Range	1.57-100ng/mL
Sensitivity	1.57ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Kinurenic acid; Kynuronic acid; Kuinurenic acid; Transtorine

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Gene Symbol

KYNA

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