General Malondialdehyde (MDA) ELISA Kit

SKU: orb781669

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Malondialdehyde(MDA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Malondialdehyde(MDA).

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Tested Applications

ELISA

Application Notes

standard: 2000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MDA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MDA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MDA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	All
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	31.25-2000 ng/mL
Sensitivity	9.47 ng/mL
Concentration	2000 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MDA

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Adam Bownik 1, Barbara Pawlik-Skowrońska 2 Effects of cyanobacterial oligopeptides: anabaenopeptin-B, aeruginosin-98B and their mixture on oxidative stress biomarkers, HSP70 production and tyrosine phosphatase activity in three aquatic invertebrate species: Brachionus calyciflorus, Thamnocephalus platyurus and Chironomus aprilinus Environ Toxicol Pharmacol, (2025)

PubMed: 40619045