General Polyethylene Glycol (PEG) ELISA Kit

SKU: orb782093

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Polyethylene Glycol(PEG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Polyethylene Glycol(PEG).

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Tested Applications

ELISA

Application Notes

standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PEG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PEG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PEG in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	All
Sample Types	biological agents
Assay Type	Competitive
Assay Time	2h
Range	15.63-1000 ng/mL
Sensitivity	4.95 ng/mL
Concentration	1000 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PEO ELISA Kit, POE ELISA Kit, Polyethylene oxide ELISA Kit, Polyoxyethylene ELISA Kit, Carbowax ELISA Kit, GoLYTELY ELISA Kit, GlycoLax ELISA Kit, Fortrans ELISA Kit, TriLyte ELISA Kit, Colyte ELISA Kit, Halflytely ELISA Kit, Macrogol ELISA Kit, MiraLAX ELISA Kit, MoviPrep ELISA Kit

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General Polyethylene Glycol (PEG) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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