General Retinol (Ret) ELISA Kit

SKU: orb781745

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Retinol(Ret) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Retinol(Ret).

Research Area

Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ret protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ret. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ret in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	All
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	156.25-10000 ng/mL
Sensitivity	44.2 ng/mL
Concentration	10000 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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VA ELISA Kit, Vitamin A ELISA Kit, Aquasola ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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