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GFAP Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Canine, Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | The immunogen for this antibody is: C-DGEVIKESKQEHKD |
| Target | GFAP |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Concentration | 500 ug/mL |
| Disclaimer | For research use only |
Alternative Names
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orb1247969 (5 ug/ml) as the reporter antibody.

orb1247969 (2 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.

orb1247969 (0.01 ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

orb1247969 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247969 (0.1 ug/ml) staining of Mouse (A) and (0.01 ug/ml) Rat (B) Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_002046.1 |
|---|
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Protocol Information
GFAP Antibody (orb1247969)
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