GFAP Rabbit Polyclonal Antibody

SKU: orb1294403

Description

Rabbit polyclonal antibody to GFAP

Research Area

Neuroscience

Images & Validation

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Item 1 of 8

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	Western Blot 1:1000-1:2000, Immunofluorescence 1:200 – 1:500, Immunohistochemistry (Frozen) 1:100 – 1:300, Immunohistochemistry (Paraffin) 1:100 – 1:300
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the C-terminus region.
Target	GFAP
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Disclaimer	For research use only

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All lanes: Anti-GFAP antibody at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose was then blocked for an hour before being incubated with orb1294403 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294403 anti-GFAP antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescent analysis. Sample: primary cortical neurons, Red: GFAP (orb1294403): 1-200, Blue: DAPI was used as the nuclear counter stain. Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical analysis of paraffin embedded Human brain tissue labeling GFAP antibody with orb1294403 at 1/100.

Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling GFAP antibody with orb1294403 at 1/100.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: GFAP (orb1294403): 1-200, Anti-rabbit 488: 1-500.

Quick Database Links

Gene Symbol

GFAP

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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