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GIPC1 Antibody

SKU: orb1247968

Description

GIPC1 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Predicted ReactivityBovine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36.0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-GAIGDAKVGRY
TargetGIPC1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

GIPC1, NIP, GIPC PDZ domain containing family, member 1, C19orf3, GIPC, GLUT1CBP, Hs.6454, IIP-1, MGC15889, MGC3774, RGS19IP1, SEMCAP, SYNECTIIN, TIP-2, GLUT1 C-terminal binding protein, IGF-1 receptor interacting protein 1, regulator of G-protein signalling 19 interacting protein 1, synectin, SYNECTIN

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GIPC1 Antibody

orb1247968 staining (0.03 ug/ml) of Human HeLa cell lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

GIPC1 Antibody

HEK293 overexpressing GIPC1 isoform 1 and probed with orb1247968 (mock transfection in first lane).

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GIPC1 Antibody (orb1247968)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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