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GLS2 Antibody
SKU: orb1239416
Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2.The immunogen is located within amino acids 300 - 350 of GLS2. |
| Target | GLS2 |
| Molecular Weight | Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa |
| Purification | GLS2 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | GLS2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GLS2 Antibody: GA, GLS, LGA, hLGA, GA, Glutaminase liver isoform, mitochondrial, L-glutaminase
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Immunofluorescence Validation of GLS2 in Mouse Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling GLS2 with orb1239416 at 20 µg g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Immunohistochemistry Validation of GLS2 in Human Liver. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLS2 antibody (orb1239416) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of GLS2 in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GLS2 antibody (orb1239416) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of GLS2 in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GLS2 antibody (orb1239416) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
WB Validation in Human Pancreas. Loading: 10 µg of lysate Antibodies: GLS2, orb1239416, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Mouse and Rat Brain. Loading: 15 µg of lysate Antibodies: GLS2, orb1239416, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GLS2 Antibody (orb1239416)
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