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GLUT2 Rabbit Polyclonal Antibody
SKU: orb183810
Featured
Description
Research Area
Cell Biology, Energy Metabolism, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Equine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human GLUT2 (1-100/524aa) |
| Target | SLC2A2 |
| Molecular Weight | 54 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GTR2_HUMAN; Solute carrier family 2, facilitated glucose transporter member 2; SLC2A2; Glucose transporter type 2, liver; GLUT-2; GLUT 2; solute carrier family 2 member 2;
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Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C). Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (orb183810), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Lane 3: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57/45 kD, Observed band size: 47 kD.
Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-GLUT2 (orb183810) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated (orb183810) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
SLC2A2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
GLUT2 Rabbit Polyclonal Antibody (orb183810)
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