Recombinant GP2 Antibody / Glycoprotein 2 / ZAP75

SKU: orb2642477

Description

GP2 (Glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

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Tested Applications	ELISA, IHC-P, WB
Dilution Range	ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant GP2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	GP2/3134R
Immunogen	A partial human recombinant protein corresponding to amino acids 35-179 was used as the immunogen for the recombinant GP2 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant GP2 Antibody / Glycoprotein 2 / ZAP75

IHC testing of FFPE human pancreas with recombinant GP2 antibody. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human pancreas lysate with recombinant GP2 antibody (clone GP2/3134R).

SDS-PAGE analysis of purified, BSA-free recombinant Glycoprotein 2 antibody (clone GP2/3134R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P55259

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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