Recombinant Glypican 3 Antibody / GPC3 / Rabbit Monoclonal

SKU: orb2640955

Description

Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt]

Images & Validation

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Tested Applications	FACS, IF, IHC-P
Dilution Range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the recombinant Glypican 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	SGPN3-2R
Immunogen	Recombinant full-length human protein was used as the immunogen for the recombinant Glypican 3 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Glypican 3 Antibody / GPC3 / Rabbit Monoclonal

IHC staining of FFPE human hepatocellular carcinoma with Glypican-3 antibody (clone SGPN3-2R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of methanol-fixed HepG2 cells with recombinant Glypican 3 antibody (green, clone SGPN3-2R) and Reddot nuclear stain (red).

Flow cytometry testing of methanol-fixed human HepG2 cells with recombinant Glypican 3 antibody (clone SGPN3-2R); Red = isotype control, Blue = recombinant Glypican 3 antibody.

Quick Database Links

UniProt

P51654

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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